Optimising glyphosate tolerance in rapeseed (Brassica napus L.) by CRISPR/Cas9-based geminiviral donor DNA replicon system with Csy4-based single-guide RNA processing Rapeseed (Brassica napus L.) is a crucial oil crop worldwide, and efficient weed management can shield its yields and high quality. Farmers can profit from tolerant cultivars when utilizing herbicides, corresponding to glyphosate. Amino acid substitutions in enolpyruvylshikimate-3-phosphate synthase […]
Computational Analysis and Validation of Potential Long Noncoding RNA
DNA and RNA Cleavage Complexes and Restore Pathway for TOP3B RNA– and DNA-Protein Crosslinks The current research demonstrates that topoisomerase 3B (TOP3B) varieties each RNA and DNA cleavage complexes (TOP3Bccs) in vivo and divulges a pathway for repairing TOP3Bccs. For inducing and detecting mobile TOP3Bccs, we engineer a “self-trapping” mutant of TOP3B (R338W-TOP3B). Transfection with R338W-TOP3B induces R-loops, genomic harm, […]
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